Larval RNAi in Drosophila?
Dev Genes Evol. 2008 Sep;218(9):505-10. Epub 2008 Jul 29.
PMID: 18663472 [PubMed - in process]
Research from Kansas State University showed some interesting finding last month. They reported that in Drosophila larvae most of tissues (except for hemocyte) were not able to uptake dsRNA, which is the reason why direct injection of dsRNA into Drosophila larvae won't trigger RNA interference (RNAi), but not due to lack of RNAi machinery.
Wow, what a surprise to me? I have been work for larval RNAi in Aedes mosquito for years by injection or feeding. Based on this article, what I did before was a waste of time. If I can read this paper long time ago, I may be able to do a lot of useful experiments and publish more paper~~ Sigh!!!
Fortunately, I am doing Tribolium now~~
